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unique dual barcodes adapters  (Illumina Inc)


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    Structured Review

    Illumina Inc unique dual barcodes adapters
    Unique Dual Barcodes Adapters, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 939 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/unique+dual+barcodes+adapters/IDT+for+Illumina+%E2%80%93+TruSeq+DNA+UD+Indexes/pmc12354490-86-17-16
    Average 99 stars, based on 939 article reviews
    unique dual barcodes adapters - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Ligation:

    Article Title: Genetic Risk Factors for Early-Onset Merkel Cell Carcinoma.
    Article Snippet: Noreen Mohsin, MD; Devin Hunt, BS, BSM; Jia Yan, MS, PhD, CGC; Austin J. Jabbour, MD; Paul Nghiem, MD, PhD; Jaehyuk Choi, MD, PhD; Yue Zhang, BSc; Alexandra F. Freeman, MD; Jenna R. E. Bergerson, MD; Stefania Dell’Orso, PhD; Kristina Lachance, MS; Rima Kulikauskas, BS; Loren Collado, BS; Wenjia Cao, MS; Justin Lack, PhD; Morgan Similuk, ScM, CGC; Bryce A. Seifert, PhD; Rajarshi Ghosh, PhD; Magdalena A. Walkiewicz, PhD; Isaac Brownell, MD, PhD

    Article Title: Personalized ctDNA micro-panels can monitor and predict clinical outcomes for patients with triple-negative breast cancer.
    Article Snippet: .. Samples were barcoded at the ligation step using Illumina unique dual barcodes adapters (Cat# 20,022,370) and were amplified for 6–8 cycles using the Library Amplification Ready-mix containing KAPA HiFi DNA Polymerase (Kapa Biosystems, Inc). ..

    Article Title: Inter and intra-host diversity of RSV in hematopoietic stem cell transplant adults with normal and delayed viral clearance.
    Article Snippet: After incubation, samples were purified with 1.8X volume AMPure XP beads and eluted into 33 μl nuclease-free water (129,114, Qiagen). .. Illumina unique dual barcodes adapters (Cat# 20,022,370) were ligated onto samples by adding 2 μl of 5uM adapter, 10 μl 5X ligation buffer and 5 μl of Expresslink Ligase (A13726101, Thermo Fisher), and incubated at 20∘C for 15 minutes. ..

    Article Title: Proteogenomic analysis of the CALGB 40601 (Alliance) HER2+ breast cancer neoadjuvant trial reveals resistance biomarkers.
    Article Snippet: For Illumina paired-end library construction, the resultant cDNA was QC’ed and processed on the Beckman BioMek FXp robots through end-repair, A-tailing and ligation of Illumina unique dual barcodes adapters (Cat# 20022370) to allow paired-end sequencing. .. For Illumina paired-end library construction, the resultant cDNA was QC’ed and processed on the Beckman BioMek FXp robots through end-repair, A-tailing and ligation of Illumina unique dual barcodes adapters (Cat# 20022370) to allow paired-end sequencing. .. The library was next amplified for 13 PCR cycles with KAPA HiFi DNA Polymerase (Kapa Biosystems, Inc., Cat # KK2612).

    Article Title: Inter and intra-host diversity of RSV in hematopoietic stem cell transplant adults with normal and delayed viral clearance
    Article Snippet: After incubation, samples were purified with 1.8X volume AMPure XP beads and eluted into 33 μl nuclease-free water (129,114, Qiagen). .. Illumina unique dual barcodes adapters (Cat# 20,022,370) were ligated onto samples by adding 2 μl of 5uM adapter, 10 μl 5X ligation buffer and 5 μl of Expresslink Ligase (A13726101, Thermo Fisher), and incubated at 20°C for 15 minutes. ..

    Article Title: Proteogenomic analysis of the CALGB 40601 (Alliance) HER2+ breast cancer neoadjuvant trial reveals resistance biomarkers
    Article Snippet: During second strand cDNA synthesis, dNTP mix containing dUTP is used to introduce strand-specificity with the NEBNext Ultra Directional RNA Second Strand Synthesis Module. .. For Illumina paired-end library construction, the resultant cDNA was QC’ed and processed on the Beckman BioMek FXp robots through end-repair, A-tailing and ligation of Illumina unique dual barcodes adapters (Cat# 20022370) to allow paired-end sequencing. ..

    Article Title: Endocrine-Sensitive Disease Rate in Postmenopausal Patients With Estrogen Receptor–Rich/ERBB2-Negative Breast Cancer Receiving Neoadjuvant Anastrozole, Fulvestrant, or Their Combination
    Article Snippet: .. Samples will be barcoded at ligation step using Illumina unique dual barcodes adapters (Cat# 20022370) and will be amplified 6-8 cycles using the Library Amplification Ready-mix containing KAPA HiFi DNA Polymerase (Kapa Biosystems, Inc). ..

    Article Title: Genetic evaluation of pediatric pituitary adenomas and USP8 -related genotype-phenotype correlations in Cushing’s disease
    Article Snippet: .. DNA end-repair and 3’-adenylation were then performed in the same reaction followed by ligation of the Illumina unique dual barcodes adapters (Cat# 20022370) to create PCR-Free libraries, and the library was run on the Fragment Analyzer (Advanced Analytical Technologies, Inc., Ames, Iowa) to assess library size and presence of remaining adapter dimers. ..

    Polymerase Chain Reaction:

    Article Title: Genetic Risk Factors for Early-Onset Merkel Cell Carcinoma.
    Article Snippet: Noreen Mohsin, MD; Devin Hunt, BS, BSM; Jia Yan, MS, PhD, CGC; Austin J. Jabbour, MD; Paul Nghiem, MD, PhD; Jaehyuk Choi, MD, PhD; Yue Zhang, BSc; Alexandra F. Freeman, MD; Jenna R. E. Bergerson, MD; Stefania Dell’Orso, PhD; Kristina Lachance, MS; Rima Kulikauskas, BS; Loren Collado, BS; Wenjia Cao, MS; Justin Lack, PhD; Morgan Similuk, ScM, CGC; Bryce A. Seifert, PhD; Rajarshi Ghosh, PhD; Magdalena A. Walkiewicz, PhD; Isaac Brownell, MD, PhD

    Article Title: Genetic evaluation of pediatric pituitary adenomas and USP8 -related genotype-phenotype correlations in Cushing’s disease
    Article Snippet: .. DNA end-repair and 3’-adenylation were then performed in the same reaction followed by ligation of the Illumina unique dual barcodes adapters (Cat# 20022370) to create PCR-Free libraries, and the library was run on the Fragment Analyzer (Advanced Analytical Technologies, Inc., Ames, Iowa) to assess library size and presence of remaining adapter dimers. ..

    Amplification:

    Article Title: Personalized ctDNA micro-panels can monitor and predict clinical outcomes for patients with triple-negative breast cancer.
    Article Snippet: .. Samples were barcoded at the ligation step using Illumina unique dual barcodes adapters (Cat# 20,022,370) and were amplified for 6–8 cycles using the Library Amplification Ready-mix containing KAPA HiFi DNA Polymerase (Kapa Biosystems, Inc). ..

    Article Title: Endocrine-Sensitive Disease Rate in Postmenopausal Patients With Estrogen Receptor–Rich/ERBB2-Negative Breast Cancer Receiving Neoadjuvant Anastrozole, Fulvestrant, or Their Combination
    Article Snippet: .. Samples will be barcoded at ligation step using Illumina unique dual barcodes adapters (Cat# 20022370) and will be amplified 6-8 cycles using the Library Amplification Ready-mix containing KAPA HiFi DNA Polymerase (Kapa Biosystems, Inc). ..

    Library Amplification:

    Article Title: Personalized ctDNA micro-panels can monitor and predict clinical outcomes for patients with triple-negative breast cancer.
    Article Snippet: .. Samples were barcoded at the ligation step using Illumina unique dual barcodes adapters (Cat# 20,022,370) and were amplified for 6–8 cycles using the Library Amplification Ready-mix containing KAPA HiFi DNA Polymerase (Kapa Biosystems, Inc). ..

    Article Title: Endocrine-Sensitive Disease Rate in Postmenopausal Patients With Estrogen Receptor–Rich/ERBB2-Negative Breast Cancer Receiving Neoadjuvant Anastrozole, Fulvestrant, or Their Combination
    Article Snippet: .. Samples will be barcoded at ligation step using Illumina unique dual barcodes adapters (Cat# 20022370) and will be amplified 6-8 cycles using the Library Amplification Ready-mix containing KAPA HiFi DNA Polymerase (Kapa Biosystems, Inc). ..

    Incubation:

    Article Title: Inter and intra-host diversity of RSV in hematopoietic stem cell transplant adults with normal and delayed viral clearance.
    Article Snippet: After incubation, samples were purified with 1.8X volume AMPure XP beads and eluted into 33 μl nuclease-free water (129,114, Qiagen). .. Illumina unique dual barcodes adapters (Cat# 20,022,370) were ligated onto samples by adding 2 μl of 5uM adapter, 10 μl 5X ligation buffer and 5 μl of Expresslink Ligase (A13726101, Thermo Fisher), and incubated at 20∘C for 15 minutes. ..

    Article Title: Inter and intra-host diversity of RSV in hematopoietic stem cell transplant adults with normal and delayed viral clearance
    Article Snippet: After incubation, samples were purified with 1.8X volume AMPure XP beads and eluted into 33 μl nuclease-free water (129,114, Qiagen). .. Illumina unique dual barcodes adapters (Cat# 20,022,370) were ligated onto samples by adding 2 μl of 5uM adapter, 10 μl 5X ligation buffer and 5 μl of Expresslink Ligase (A13726101, Thermo Fisher), and incubated at 20°C for 15 minutes. ..

    Sequencing:

    Article Title: Proteogenomic analysis of the CALGB 40601 (Alliance) HER2+ breast cancer neoadjuvant trial reveals resistance biomarkers.
    Article Snippet: For Illumina paired-end library construction, the resultant cDNA was QC’ed and processed on the Beckman BioMek FXp robots through end-repair, A-tailing and ligation of Illumina unique dual barcodes adapters (Cat# 20022370) to allow paired-end sequencing. .. For Illumina paired-end library construction, the resultant cDNA was QC’ed and processed on the Beckman BioMek FXp robots through end-repair, A-tailing and ligation of Illumina unique dual barcodes adapters (Cat# 20022370) to allow paired-end sequencing. .. The library was next amplified for 13 PCR cycles with KAPA HiFi DNA Polymerase (Kapa Biosystems, Inc., Cat # KK2612).

    Article Title: Proteogenomic analysis of the CALGB 40601 (Alliance) HER2+ breast cancer neoadjuvant trial reveals resistance biomarkers
    Article Snippet: During second strand cDNA synthesis, dNTP mix containing dUTP is used to introduce strand-specificity with the NEBNext Ultra Directional RNA Second Strand Synthesis Module. .. For Illumina paired-end library construction, the resultant cDNA was QC’ed and processed on the Beckman BioMek FXp robots through end-repair, A-tailing and ligation of Illumina unique dual barcodes adapters (Cat# 20022370) to allow paired-end sequencing. ..



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